(C) Nterminally HAtagged wildtype (WT) FUS and the indicated point mutants were transiently expressed in HeLa cells; 24 h posttransfection cells were stained with an HAspecific antibody (green), a nuclear counterstain (blue) and analysed by confocal microscopy. Although the WT protein is located almost exclusively in the nucleus, deletion of the Cterminal 13 amino acids ( or replacement of arginine residues by alanine (R521A/R522A/R524A or R514A/R518A) leads to a predominantly cytosolic localization. Scale bar, 20 (D) Quantification of nuclear and cytosolic fluorescence intensities.Eli Manning likes this stage. With that many weapons, you can't bet against him. GIANTS 34, PATRIOTS 20.

It's just a matter of trying to pick it up. I've played in a variety of different systems. It's just a matter of putting it all together.".12. I mean seriously, if the Bills defense gives up an average of 170 rushing yards per game, why wouldn't you choose to rush Maroney 20 times and Sa