To determine if unlabelled cells in the above fluorescence assay had accumulated peptide below detectable levels, we next evaluated peptide incorporation using the more sensitive fluorescenceactivated cell sorting (FACS) technique. Cells were incubated for 1 hour with media containing increasing concentrations of carboxyfluoresceintagged peptide. To discriminate between potentially nonspecific membranebound versus internalized fluorochrome, peptidetreated cells were incubated with trypsin prior to FACS analysis.21 At 10 of carboxyfluoresceintagged peptide, 95.4 3.3 % of 9L and 32.7 4.3 % of F98 cells internalized the peptide, while at 20 most glioma cells internalized the peptide (100 0.3 % of 9L and 72.1 6.9 % of F98 glioma cells).Names like Ray Lewis, Ed Reed, and Anquan Boldin are gone from the roster. Most of the secondary has changed; along with pass rush special Paul Kruger leaving. This preseason has seen starting tight end Dennis Pitta go out injured for the year.